Select the Task for primer selection




Paste source sequence below (5'->3', string of ACGTNacgtn -- other letters treated as N -- numbers and blanks ignored). FASTA format ok. Please N-out undesirable sequence (vector, ALUs, LINEs, etc.) or use a Mispriming Library (repeat library)

Pick left primer, or use left primer below Pick hybridization probe (internal oligo), or use oligo below Pick right primer, or use right primer below (5' to 3' on opposite strand)
Left Primer Sequence Input:
Right Primer Sequence Input:
Oligo Primer Sequence Input:

Sequence Id
Targets
Overlap Junction List
Excluded Regions
Pair OK Region List
Included Region
Start Codon Position
Internal Oligo Excluded Region

Sequence Quality

PCR Primer 设计基础条件设置:


  Min Opt Max
Primer Size
Primer Tm
Product Tm
Primer GC%

Product Size Ranges
Number To Return Max 3' Stability
Max Library Mispriming Pair Max Library Mispriming


单条引物罚分权重参数设置:


Tm Lt: Gt:
Size Lt: Gt:
GC% Lt: Gt:

TH: Self Complementarity
TH: 3' End Self Complementarity
TH: Hairpin
TH: Template Mispriming

Self Complementarity
3' End Self Complementarity
Template Mispriming

#N's
Library Mispriming
Sequence Quality
End Sequence Quality
Position Penalty
End Stability
Primer failure rate

成对引物罚分权重参数设置:


Product Size Lt Gt
Product Tm Lt Gt

TH: Any Complementarity
TH: 3' End Complementarity
TH: Template Mispriming Weight

Any Complementarity
3' End Complementarity
Template Mispriming Weight

Tm Difference
Library Mispriming
Primer Penalty Weight
Internal Oligo Penalty Weight




温馨提示:

此在线工具可以为您的引物设计提供一些便利,所设计引物必须自行到NCBI数据库检查特异性或其他技术指标。对于特殊基因、物种或复杂情形,此版本可能无法满足您全部设计需求,如遇到合成失败或特异性不足等问题,本软件将不承担此风险,最终解释权归锐博生物所有。如需委托锐博生物做引物设计及合成,请您到本网站“设计定制类产品”中填写设计需求并提交订单。